Lentiviral expression system for the purification of secreted proteins from human cell cultures
نویسندگان
چکیده
منابع مشابه
Lentiviral expression system for the purification of secreted proteins from human cell cultures
BACKGROUND Recombinant proteins of therapeutic use are ideally produced in human cells to ensure appropriate co- and post-translational modifications. However, purification of secreted proteins from the culture media is impeded by low expression from transfected cell lines and the presence of serum proteins. Here we describe a simple and cost-effective approach based on lentiviral vector-mediat...
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ژورنال
عنوان ژورنال: BMC Biotechnology
سال: 2016
ISSN: 1472-6750
DOI: 10.1186/s12896-016-0288-3